Montag
05
JUL

17.00 Uhr

Colloquium: Engineering Molecular Systems

Nanostructures for Probing and Transfecting Living Cells

Prof. Dr. Christelle Prinz, Lund University, Solid State Physics, Sweden

Interacting with cells in a minimally invasive manner is key to performing longitudinal studies in biomedicine. One example where minimally invasive interaction with cells is important is cellular mechanosensing. Mechanical forces are exerted in many crucial cellular processes and the investigation of cellular forces is expected to yield important information on, e.g., cancer formation and epithelial to mesenchymal transition (metastasis). In order to extract useful information from force measurements, one must be able to measure traction forces on multiple cells, over long

periods of time without any detrimental effects on the cells. This can be achieved by using nanowires in a bed-of-nails configuration, measuring traction forces exerted by cells cultured on top of the nanowires.

Another type of intervention consists of injecting material inside cells, either to probe cells, or to change the cell phenotype (transfection). Current delivery methods such as viral vectors electroporation and liposome delivery (lipofection) have many drawbacks. Viruses suffer from being costly, can be unsafe due to insertional mutagenesis, and are limited in cargo size. Electroporation and lipofection, suffer from low cell viability and efficiency. To address this issues, nanostraws can be used to deliver cargos inside cells.

Here, a review of our work using nanowires and nanostraws for developing mechanosensors and cell transfection tools will be presented.

Adresse

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Homepage Veranstaltung

https://www.uni-heidelberg.de/de/node/32968

Veranstalter

Flagship-Initiative Engineering Molecular Systems

Homepage Veranstalter

https://www.uni-heidelberg.de/de/forschung/forschungsprofil/exzellenzstrategie/engineering-molecular-systems

Kontakt

Dr. Fania Geiger

Alle Termine der Veranstaltung 'Colloquium: Engineering Molecular Systems':

Organised by the Flagship Initiative Engineering Molecular Systems, the FI EMS Colloquium presents the different areas of Molecular Systems Engineering. The speakers include external experts and young scientists of Heidelberg University.

Montag, 03. Mai 2021, 17.00 Uhr

Molecular Engineering of Peptide Conjugates for Cardiovascular Therapies

Prof. Dr. Matthew Tirrell, The University of Chicago (USA), Pritzker School of Molecular Engineering

Montag, 05. Juli 2021, 17.00 Uhr

Colloquium: Engineering Molecular Systems

Prof. Dr. Christelle Prinz, Lund University, Solid State Physics, Sweden

Montag, 20. September 2021, 17.00 Uhr

Engineering synthetic T cell platforms for cancer immunotherapy

Prof.Dr. Sai Reddy, ETH Zürich , Switzerland

Montag, 11. Oktober 2021, 17.00 Uhr

Printing Functional Soft Materials: Towards Life-like Behaviors

Jun.-Prof. Eva Blasco, Universität Heidelberg, Organisch-chemisches Institut

Montag, 06. Dezember 2021, 17.00 Uhr

Soft x-ray microscopy of single cells

Dr. Venera Weinhardt, Heidelberg University, Centre for Organismal Studies